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Alberti KGMM, Eckel RH, Grundy SM, Zimmet PZ, Cleeman JI, Donato KA, Fruchart JC, James WPT, Loria CM, Smith SC

Nonmigrated cells were removed with cotton swabs, and migrated cells were fixed with ice-cold methanol and stained with 4,6-diamidino-2-phenylindole (DAPI)

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Three primary reasons drive the logic behind peptide rotation in research contexts: 1

For 96-well plates, 10 L of prepared reagent (1:50 ratio of monochlorobimane (MCB) to Tris assay buffer, per manufacturers instructions) was added to wells containing 100 L of culture media and incubated for 30 min at 37 C, washed with PBS, and imaged using an Olympus IX70 microscope with a DAPI filter (Excitation/Emission: 358 nm/461 nm)